Diagnosis of Streptococcus canis periprosthetic joint infection: the utility of next-generation sequencing

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Diagnosis of Streptococcus canis periprosthetic joint infection: the utility of next-generation sequencing

A 62-year-old man who had undergone a primary knee arthroplasty 3 years earlier, presented to the emergency department with an infected prosthesis. He underwent prosthesis resection. All cultures failed to identify the infecting organism. Analysis of the intraoperative samples by next-generation sequencing revealed Streptococcus canis (an organism that resides in the oral cavity of dogs). It wa...

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Diagnosis of Periprosthetic Joint Infection: The Potential of Next-Generation Sequencing.

BACKGROUND Next-generation sequencing is a well-established technique for sequencing of DNA and has recently gained attention in many fields of medicine. Our aim was to evaluate the accuracy of next-generation sequencing in identifying the causative organism(s) in patients with periprosthetic joint infection. METHODS In this prospective study, samples were collected from 65 revision arthropla...

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Prevention of Periprosthetic Joint Infection

Prosthetic joint infection (PJI) is a calamitous complication with high morbidity and substantial cost. The reported incidence is low but it is probably underestimated due to the difficulty in diagnosis. PJI has challenged the orthopaedic community for several years and despite all the advances in this field, it is still a real concern with immense impact on patients, and the healthcare system....

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Strategies and Clinical Applications of Next Generation Sequencing

Abstract DNA sequencing is one of the great valuable techniques in molecular biology, which can be used to detect the sequence of nucleotides in a DNA fragment. The high-throughput se­quencing known as Next Generation Sequencing (NGS) revolutionized genomic research and molecular biology; therefore, the whole human genome can be sequenced with a low cost in several days. NGS technology is simi...

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ژورنال

عنوان ژورنال: Arthroplasty Today

سال: 2018

ISSN: 2352-3441

DOI: 10.1016/j.artd.2017.08.005